Dvořák wrote it in the summer of 1893, during his years running the National Conservatory in New York, while on holiday in Spillville, a Czech-speaking village in Iowa. He sketched it in three days and finished it a little over two weeks after starting.
Much of the melody is built on the five-note (pentatonic) scale, which gives the whole quartet its open, folk-like sound. The first public performances were by the Kneisel Quartet, in Boston on January 1, 1894, and in New York twelve days later.